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Edges may simply reflect binary data (presence or absence of a ties, e.g. groomed or did not groom) or they may reflect continuous data that reflects the frequency or strength of the interaction (e.g. grooming frequency or duration).
The two species with naked pupae showed no significant differences in grooming frequency between sham-treated and dead or live fungus-exposed brood.
Grooming frequency may be a crude measure for bondedness, but previous analyses using grooming diversity/distribution did not find any connection with inter-group conflict either., My analysis could be repeated with social network measures such as group density, a measure of group cohesiveness.
Nil responses (data not shown) were observed for the number of attempted mounts, intromission latency, ejaculation latency, intromission frequency, ejaculation frequency, postejaculatory interval, number of thrusts per intromission, and grooming frequency, as previously described (Mani et al. 2005; Markaverich et al. 2002a, 2002b).
In contrast, the two species with cocooned pupae groomed live fungus-exposed brood significantly more than sham-treated brood, and showed a near-significant trend for higher grooming frequency of brood treated with live vs dead fungus.
They found levels of relationship satisfaction and trust were both positively correlated with self-reported grooming frequency among romantic partners.
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Neither pupal type (naked vs cocooned; LMM, F1,32 = 1.842, P = 0.184) nor brood treatment (sham control, dead or live fungus; F2,32 = 1.144, P = 0.331) significantly affected grooming frequencies in F. selysi.
Sexual behavior was evaluated by examining the number of mounts, intromissions, ejaculations, ejaculation latencies (in seconds), and grooming frequencies in a 30-min test period with each sexually receptive female as per well-established procedures in our laboratories (Hull et al. 1990).
In the breeding period, mating frequency correlated with the frequency of grooming directed by males to females (MF grooming; Kr = 26, τKr = 0.609, P = 0.001, α = 0.0125 adjusted via Bonferroni) but not with the frequency of grooming performed by females to males (FM grooming; Kr = 12, τKr = 0.336, P = 0.091).
Following 1 hr immobilization stress exposure, the changes of frequency in grooming bouts and total time in grooming durations were tested in each genotype.
The frequency of grooming behaviors was counted in a 10 min observation period.
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