Sentence examples for grinding tissue from inspiring English sources

Spleen and lymph node cell suspensions were prepared by grinding tissue through sterile mesh and erythrocytes were lysed with RBC lysis buffer (eBioscience, CA).

ATP was extracted in 1 mL of 3% trichloroacetic acid by grinding tissue (150 mg) in liquid nitrogen.

RNA was prepared by grinding tissue in liquid nitrogen and extracting using TRI Reagent (Sigma-Aldrich, St . Louis MO, USA) according to the manufacturer's protocol.

Our data suggests that grinding tissue to a powder in liquid N2 may be insufficient to ensure complete homogenization and random sampling from a large structure.

After a 2 hour treatment leaves were patted dry and RNA was extracted by flash freezing and grinding tissue in liquid nitrogen prior to extraction using a Qiagen RNeasy kit.

Samples were extracted by grinding tissues in liquid nitrogen followed by re suspension in Qiazol (Qiagen).

Protein extracts were prepared by grinding tissues on ice in extraction buffer (5% glycerol, 4% SDS, 1% polyvinylpolypyrrolidone, 1 mM phenylmethylsulfonyl fluoride, 50 mM Tris, pH 8.0), followed by centrifugation at 4°C and 14,000 g for 15 min.

The yields of exposed plants to pure chemicals (Andersen 1981), ground tissue of allelopathic plants (Tseng et al. 2003) and soil mediated allelopathic plants (Weidenhamer et al. 1989) are consistent with the assumptions of a density-dependent phytotoxicity model.

Also, vascular and ground tissue disintegration occurred [Figure 2e, 2f].

This result is consistent with the observations that transcripts of the cab genes are not detectable in the below ground tissue by Northern blot hybridization (Fig. 2).

Total RNA was extracted from liquid nitrogen frozen and ground tissue using the miRVana miRNA extraction Kit (Ambion) and Plant Isolation Aid step (Ambion) according to the manufacturer's instructions.