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Total RNA from tissue was isolated after homogenization with a Braun Mikrodismembranator. Cell aggregate cultures were ground up in the Tri-Reagent with Molecular Grinding Resin (Geno Technology Inc., St Louis, MO, USA).
After 14 days, cell-seeded collagen hydrogels and pellets were disrupted with Molecular Grinding Resin in TRIzol (Geno Technology Inc., St . Louis MO, USA), and RNA was extracted according to the manufacturer's recommendations.
Total RNA was extracted from pellets using Trizol (Invitrogen) after grinding with Molecular Grinding Resin (Geno Technology Inc. St Louis, USA) in combination with the use of RNeasy mini kit (Qiagen, Mississauga, Ontario, Canada) and after removal of contaminating genomic DNA from the pellets by DNase treatment.
Total RNA was extracted from pellets by using Tri-Reagent (Sigma-Aldrich) after grinding with Molecular Grinding Resin (Geno Technology Inc., St . Louis MO, USA) in combination with the use of an Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Mississauga, ON, Canada) and after removal of contaminating genomic DNA from the pellets by DNase treatment.
Total RNA was extracted with Tri Reagent (Sigma) from passage 2 cells in monolayer and from cell aggregates at 14 days that had been ground with Molecular Grinding Resin (Geno Technology Inc., St Louis, MO, USA).
Total RNA was extracted with Tri Reagent (Sigma-Aldrich) from passage 2 cells in monolayer and from cell aggregates at 14 days which had been ground with Molecular Grinding Resin (Geno Technology Inc., St . Louis MO, USA).
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Briefly, the samples were homogenized in the sample grinding tube with abrasive resin by using the provided plastic pestles.
The supply is limited, and it has other uses in glass, in enamels, and as a filler for resin-bonded grinding wheels.
For grinding applications, softer binders, e.g. resin, porous ceramics, and soft metals, are used.
Whole muscle extracts were prepared from individual samples by grinding the quadriceps with an abrasive resin (PlusOne Sample Grinding Kit, Amersham Biosciences) in urea/CHAPS buffer (8M urea, 4% CHAPS) following the manufacturer's recommendations.
Then, the fluid exudate was subjected to drying at 40 °C followed by grinding to yield 24 g of dried resin.
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