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In this study we investigate the effect of GRIN system length on the imaging performance.
Figure 3 shows the most and least uniform intensity curves obtained from the transmission imaging (GRIN system 1B and 2C respectively).
Use of a small diameter lens to penetrate deeply within tissue has been demonstrated with a hypodermic needle GRIN system [ 24].
M. falcata seeds were obtained from the Agricultural Research Service ARSS) GRIN system (http://www.ars-grin.gov/), US Department of Agriculture (USDA).
We characterize the loss in imaging performance with increased GRIN system length and present a compact and portable two-photon microscope that could be used in a clinical environment in combination with various GRIN lens systems.
The on-axis lateral and axial resolutions and FOV of the systems are summarized in Table 1. Figure 2 shows the beam profile along the axial and lateral directions of the longest GRIN system (2C).
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aFive different doublet GRIN systems were designed by and purchased from GRINTECH GmbH.
Previous studies, however, have been limited to short (<4 cm) GRIN systems primarily intended for small animals and external clinical use [ 19– 23].
These results indicate that the off-axis resolution of these GRIN systems remains within ~20% of the on-axis resolution for most of the FOV (~80% of the area).
However, alternative analyses can be performed using this dataset, which is publicly available through the T3 database (http://triticeaetoolbox.org/) and the ARS-GRIN system (http://www.ars-grin.gov/).
The effect of the longest GRIN lens system (285 mm in length, system 2C) on the excitation laser pulse was characterized.
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