The grids were visualized using an H600 transmission electron microscope (Hitachi, Chiyoda-ku, Japan) with an operating voltage of 60 kV.
After negative staining with uranyl acetate and lead citrate for 5 min, the grids were visualized using an H600 transmission electron microscope (Hitachi Ltd ,Chiyoda-ku, Japan) with an operating voltage of 60 kV.
After drying, the grids were visualized on a JEOL JEM 1200 EXII transmission electron microscope.
All statistical analyses were performed using R [63]; deformation grids were visualized with the tpsgrid function of the R shape package [60].
Grids were visualized using a Hitachi-7100 transmission electron microscope running at 100 kV.
For EM, 5 μl of the VLDL preparation was applied to carbon-coated copper grids and stained with 2.0% uranyl acetate for 15 min. Grids were visualized with a JEOL 100CX transmission electron microscope.
Stained samples on grids were visualized using a JEOL 1400 TEM and digital micrographs of individual cells were acquired at × 4000 magnification with Gatan Digital Micrograph software (Pleasanton, CA, USA).
Grids were visualized with an FEI Tecnai 12 transmission electron microscope operating at 120 kV, and images were recorded on Kodak SO-163 film at 30000× nominal magnification or on a Gatan Ultrascan 1000 CCD camera at 42000× nominal magnification.
The existence of problems or findings was visualized on a timeline grid which is a collection of explicit date cells.
Variation in shape was visualized using thin-plate spline transformation grids in tpsRegr (Rohlf 2004d).
