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An example is the four-electrode tube known as the tetrode, in which an open-mesh grid (screen grid) maintained at a positive voltage is placed between plate and control grid.
The initial crystallization conditions were further optimized using a grid screen by varying the concentration of the precipitant.
Commercially available sets of solutions including the HR-110 Crystal Screen kit, the HR-112 Crystal Screen II kit, the PEG/LiCl screen, and Grid Screen Polyethylene Glycol 6000 (Hampton Research Co). were also tested.
However, the MPD-pH grid screen provided some interesting results.
All screen solutions contained 2 mM GSH. (i) MPD - Fine pH Grid Screen.
We also explored the dependence of crystal growth on CR-concentration by performing a pH versus CR-concentration grid screen of hemoglobin and Pin1R14A on one of the unique CR-hits.
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Preliminary results regarding cycle time, failure rates, positioning accuracy and a 96 grid screening experiment are presented.
Initial crystallization hits were identified using the MCSG2 sparse matrix screen (Microlytics) and subsequently optimized by grid screening.
Initial grid screening is done at ultra low dose rates (<10−6 e Å−2 s−1) and low magnification (∼100×) in bright field (Fig. 1 ▸ a).
Crystals of Cv1693 from the original crystallization attempts were thin spindly needles that did not improve in shape upon standard grid screening protocols.
Instead of sealing up the final section of screening, use 1/4-inch (6.35-mm) grid screening to form a funnel.
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