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The reassortant virus grew efficiently in MDCK cells, reaching a titer of 8.7 log10Tcomparablecomparable to the titer of the parental H3N2 virus (8.4 log10TCID50/ml) and higher than the parental H9N2 virus (7.2 log10TCindicatingindicatingoodod compatibility of the gene constellation for this reassortant virus.
However, fspA KO cells grew efficiently in the presence of LM21, a capsulated strain of K. pneumoniae.
Consistent with previous findings [ 5], Control-IEC-6 cells failed to grow in soft agar whereas Tpr-Met-IEC-6 cells grew efficiently, forming numerous large colonies.
Both grew efficiently in this cell line with an evident CPE noted after a mean incubation time of 3.8 days (range 1 8 days).
In this experiment, wild-type Ad26 and rcAd26.dE3.empty grew efficiently in A549 cells infected with an MOI of 1,000 or 100, whereas the replication-incompetent Ad26 vector did not.
In a single-step growth assay (Fig 8a,b), bM24-CMV grew efficiently in Vero cells, with slower kinetics than hrR3 or KOS, presumably reflecting the lower and slower induction of ICP4 by the CMVIE promoter.
Similar(47)
How could we grow efficiently and sustainably?
Though I have, obviously, found a way to ignore it.* In vitro meat heralds the dawn of a new age where oodles of the stuff can be grown efficiently and the world fed painlessly (if we gloss over its culturing in antibiotics and – displacing "carpet tiles" as the world's unloveliest phrase – something called foetal bovine serum).
Microalgae are in fact, capable of growing efficiently on many types of wastes/wastewaters.
A strain of At that grows efficiently on rice straw for cellulase production was isolated, identified and characterized.
Managing these new risks and systems is a must when trying to grow efficiently within the platform ecosystem.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com