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But even as he grew cells in dishes, he was experimenting with growing things on a larger scale in the desert.
"I grew cells," he said.
We grew cells in DMEM (Invitrogen) containing 10% FCS, 1X essential amino acids (Gibco), pen/strep, and gentamicin.
We grew cells at low temperature (22°C), since at 28°C no structures were observed in any combination (not shown).
To test fluorescence activity, we grew cells containing Gemini, GFP, and α-fragment expression constructs with promoter J23101 in supplemented M9 media at 37°C.
Basically, we grew cells in YE media to an OD600 of 0.3 at 30°C under continuous shaking in Erlenmeyer flasks.
Similar(30)
It turned out that I had rapidly growing cells.
These include biochemicals, nutrients for growing cells, glassware and sterile liquid-handling systems.
Wine and biotech drugs are both made by growing cells, usually in stainless-steel tanks.
Nexell Therapeutics of Irvine, Calif., also has techniques for separating and growing cells.
Growing cells from the epidermis, the outermost layer of skin, is both tricky and time consuming.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com