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The percentage of the cotyledon expansion and greening seedlings were measured.
GFP-CAO fusion transgenes were highly overexpressed in the etiolated and greening seedlings of all transgenic tGABC ch and tGBC ch plant lines (Fig. 2A).
In contrast, the full-length CAO (A-B-C domains) and the GFP-full-length CAO (a fusion of GFP and A-B-C domains of CAO) proteins were under a detectable level by immunoblotting in both etiolated and greening seedlings (Fig. 2B).
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4°C/7 d 25°C/10 d (Xie et al. [2012]) Seedling growth 1 = dark green seedlings, 3 = light green seedlings, 5 = yellow seedlings, 7 = brown seedlings, 9 = seedlings dead.
This is the season for winter wheat, the time when lush, green seedlings usually blanket the earth.
The tiny, gray-green seedlings don't appear until well after the weather has warmed, but once they do show up, they're patient.
And they dig into the ground with their spading forks every summer so that tiny green seedlings can grow into beefy tomatoes and sweet red peppers by fall.
The danger is that sparking seeds into life too early by giving them heat before you can also offer the necessary light levels results in leggy, pale specimens rather than the meaty green seedlings that make good productive plants.
Effects of simulated microgravity and hypergravity on the senescence of oat leaf segments excised from the primary leaves of 8-d-old green seedlings were studied using a 3-dimensional (D) clinostat as a simulator of weightlessness and a centrifuge, respectively.
After 14 d of cold treatment at 9°C, this assay is performed by scoring seedlings as 1 = dark green seedlings, 3 = light green seedlings, 5 = yellow seedlings, 7 = brown seedlings, 9 = dead seedlings (Kim and Tai [2011]; Andaya and Tai [2006]).
The surviving green seedlings were considered to be transgenic lines that were resistant to hygromycin, whilst those wilting or undeveloped protocorms were hygromycin susceptible wild-type like progenies.
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