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For this test, we specified Sciaphila as the foreground lineage and all green taxa as background lineages.
The rps7 locus of Sciaphila (ωMHT = 0.611) approached the expectations for neutral evolution (ω ≈ 1), compared with evidence of strong purifying selection (ω = 0.203) in green taxa.
The two-ratio model (M1) was a better fit for clpP, rpl14, and rps7 after Bonferroni correction, suggesting that these genes are under a significantly different selective regime in Sciaphila than in the green taxa.
In the simplest model (M0, one ratio), all branches evolve under one ω-ratio (i.e., ωMHT = ωgreen; see supplementary table S3, Supplementary Material online. In the alternative model (M1, two ratios), Sciaphila was allowed to evolve under a different ω-ratio than the green taxa (i.e., two ratios allowed, ωMHT and ωgreen).
Although we detected significant differences in ω rates for clpP and rpl14, these two genes are still predicted to be experiencing purifying selection in Sciaphila, although this may have also been relaxed (ωMHT = 0.288 and ωMHT = 0.240, respectively, for clpP and rpl14 in Sciaphila; the corresponding values for green taxa are: ωgreen = 0.154 and ωgreen = 0.096).
Here, we report on full plastid genomes and plastid gene sets recovered from Sciaphila densiflora (Triuridaceae) and ten related green taxa in Pandanales and Dioscoreales (comprising complete plastid genomes for Sciaphila and a green relative, Carludovica palmata, and plastid gene sets for nine additional relatives).
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The names referring to the yellow-green taxa were usually treated as synonyms of Acarospora smaragdula (e.g. Purvis et al., 19851987; Santesson et al., 2004).
The chloroplast genomes of 63 green algal taxa were used in the phylogenomic analyses.
Mitochondrial genome sequences were retrieved from GenBank for the 25 green plant taxa listed in table 1.
Driskell et al. [ 6] mined GenBank to build a sparse data set of 251 loci and 69 green plant taxa with a coverage density of 15% (Table 1).
We assembled protein-coding sequence data for 78 genes from 360 diverse green plant taxa with complete or nearly complete plastid genome sequences available from GenBank.
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