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There was always this distinctive green staining of the cess, but no smell after 2,000 years.
Note that AURORA B (d, green staining) and SURVIVIN (f, green staining) are concentrated at the cleavage furrow and the lagging kinetochore (white arrows in d and e).
Panels in (a) show laminin (green staining; upper panels) and collagen-1 (green staining ; lower panels) production in NP-treated cells.
Amplification was analyzed by using 2% agarose gel electrophoresis and ethidium bromide and SYBR Green staining.
Histological examination by hematoxylin eosin and safranin O/fast green staining indicates that the cells regained their original round morphology.
Red staining indicates expression of RAGE, yellow staining indicates ZO-1 expression, and green staining indicates expression of SP-D.
Note that AURORA A (green staining) is localized to all three spindle poles (white arrows in b).
The safranin O/fast green staining showed that the central part was positively stained for GAG while the periphery was negatively stained.
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Quantitative PCR was performed using the Lightcycler system (Roche Diagnostics, Mannheim, Germany) with SYBR-green staining of DNA double-strands.
The SYTOX Green staining experiment indicated that the system can be used to induce cell death by green-light exposure.
The cDNA corresponding to 50 ng total RNA was used for real-time PCR using a SYBR Green staining method (ABI StepOne™ Real-Time PCR system, Applied Biosystems).
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