Sentence examples for green sequence from inspiring English sources

Each PCR reaction contained 10 ng first-strand cDNA (prepared from each mRNA sample using Superscript™ reverse transcriptase, Life Technologies, Carlsbad, CA, USA), SYBR green sequence detection reagents (Applied Biosystems, Foster City, CA, USA), and sense and antisense primers.

By contrast, the miR399 5p sequences (green sequences in Figure  1) only share three conserved nt.

Sequences were cleaned using Wizard(r ) Magnesil Green Sequencing Reaction Clean-Up System (Promega) and analyzed with an ABI 3730.

First, reads are sorted according to the primer sequences used to target each locus: the yellow and blue sequences represent the primers in the 3', respectively 5' flank of the first targeted L1 (locus 1) whereas the red and green sequences represent the primers in the 3', respectively 5' flank of the second targeted L1 (locus 2).

Structure of RNAP (gray ribbons; black circle for active-center region; violet sphere for Mg2+(I); β' non-conserved region and σ omitted for clarity; Mukhopadhyay et al., 2008 ), showing sites of GE-resistant substitutions (green; sequences from A and Supplementary file 2C ).

A well-supported clan contains a clan comprising all non-plant sequences, the green algal sequence from V. carteri, and several angiosperm clans.

For the large tree the green alga sequence OtWOX was used as outgroup, whereas the fern sequence CrWOXA was used as outgroup for the intermediate clade and modern clade sub-trees.

In addition, SYBR Green I sequence specificity was investigated, using a set of sequences differing in length and base composition.

Two eukaryotic groups are found in the GDH-2 phylogenetic tree, one cluster with two green algal sequences and an Arabidopsis sequence, and a second larger cluster.

Table 2 Means and standard errors (SE) of cumulative CH4 emissions (kg CH4-C ha−1) of a grass-clover – silage maize – green manure sequence.

Table 1 Means and standard errors (SE) of cumulative area-scaled N2O emissions (kg N2O-N ha−1) of a grass-clover – silage maize – green manure sequence.