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Representative traces (right) of current-clamped DRG cells firing action potentials in response to current injections (blue bars) and 532-nm green light pulses (green pulse).
Glowing fields of yellow and green pulse up her body.
We stimulated pyramidal cells with a blue light pulse followed by a green pulse after 3 s, which reduced the photocurrents to 9.4±1.2% (n = 24; Fig. 2B).
To minimize current loss between stimulation trials, light-induced depolarization was terminated by applying a green pulse 20 s after stimulation.
Dilutional methods to measure cardiac output include the transpulmonary lithium and indocyanine green (pulse dye) techniques, allowing peripheral injections and peripheral and, for pulse dye, non-invasive detection.
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Here, the record of that green, pulsing jungle life is boxed and stacked in plain gray cartons, arranged in towers that reach the ceiling.
Light pulse power (measured in the back focal plane of the objective) was 8.4 mW for blue and 44 mW for green pulses, if not indicated otherwise.
(b) The repetitive dynamical nuclear polarization cycle: (1) the NV centers are initialized by a green laser pulse; (2) a ((frac{pi}{2})_{y}) pulse with the frequency (omega(t)) prepares the NV centers into the dressed spin down state; (3) the frequency-modulated microwave fields are applied to facilitate the polarization transfer from the NV center to the nuclei.
Next, we tested whether the number of spike trains fired by a cell could be increased by interrupting the light-triggered depolarization using a green light pulse applied 20 s after each blue pulse (Fig. 3C).
Application of the green light pulse 20 s or 60 s after the blue stimulation pulse was less effective in preventing photocurrent reduction.
As the NV center spin can be re-polarized by a green laser pulse, the polarization transfer process can be repeated, see Figure 1(b), and thus allowing us to achieve high efficiency dynamical nuclear polarization [29].
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