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Completely green protocol was developed for their synthesis.
The current study presents an effective and new green protocol for the synthesis of thiophene-appended pyrazoles through 3 + 2 annulations method.
A green protocol for the synthesis of quinazoline-2,4(1H,3H -diones from carbon dioxide and 2-aminobenzonitriles using a basic ionic liquid, 1H,3H -dioneshyl imidazolium hydroxide ([Bmim]OH) as a catalyst have been reported.
"This will not solve the problem of migration — there will still be a social problem," Marcos Jank, the president of the association, told a briefing on biofuels in Sao Paulo, adding the group had signed a new "social" and "green" protocol with the government to improve overall conditions in the field.
This green protocol does not require any additive and the photocatalyst is abundant and recyclable, providing a yield up to 64% and >20 1 diastereoselectivity. Mechanistic investigations were carried out by using product analysis, kinetic studies, O-18 labelling experiments, electron-spin resonance and a set of quenching experiments.
The quantitative reverse transcription PCR of ABCG2 and BAX and BCL2 was performed according to the SYBR green protocol (Amplicon, Denmark).
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CuNPs with different structural properties and effective biological effects can be fabricated using new green protocols in the coming days.
Although all these green protocols for CuNP synthesis have their own advantages and limitations, the use of plant extract as a reductant is more beneficial as compared to the use of microbial extract because of the rapid rate of production of nanoparticles with former green reductant.
miRNA expression levels were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR), using Sybr Green protocols and commercially available assays (Qiagen) on an Applied Biosystems 7900HT instrument.
Taqman or SYBR green protocols were used to amplify cDNAs of interest by real-time quantitative PCR (Q-PCR) using a Rotor-Gene 3000 (Corbett Research, Sydney, NSW, Australia).
On the basis of extensive docking experiments, ten promising compounds out of 55 initially taken were synthesized using green protocols and their in vitro anti-HIV activity assessed in TZM bl cells by luciferase assay and reverse transcriptase (RT) inhibition assay against wild type HIV-1 RT.
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