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Inorganic phosphate released from ATP was measured using the Malachite green phosphate detection kit (R&D Systems, Wiesbaden-Nordenstadt, Germany).
The reaction was terminated by the addition of malachite green phosphate detection solution for 10 15 min at room temperature, and free phosphate was quantified by measuring the absorbance of the mixture at 650 nm using a microplate reader.
ATPase and GTPase activities were determined using "malachite green phosphate assay kits" from BioAssay Systems.
Malachite green phosphate assays were performed according to the manufacturer's instructions (Abnova).
The release of inorganic phosphate was determined using a malachite green phosphate detection kit (Innova).
The phosphate content of serum was determined with Malachite Green Phosphate Assay kit (BioAssay Systems, Hayward, CA).
Similar(50)
The amount of phosphate released was measured by the absorbance of the molybdate-malachite green-phosphate complex at 630 nm.
The partition coefficient of Lysosensor Green between phosphate buffered saline (PBS) (pH = 7.4) and poly (propylene oxide) (PPO, MW = 4500 g/mol, Sigma Chemicals) was determined using the fluorescent characteristics of the probe.
Prior to the malachite green free phosphate assay, the GST-Abl solution was concentrated to about 0.5 μg/μl with an Amicon Ultra-4 Centrifugal Filter (10 K, Millipore).
"The detergent industry realized they were going to face a patchwork of state laws," says Martin Wolf, director of product and environmental technology for Seventh Generation, which has made a "green" non-phosphate dishwasher detergent since 1997.
(A) Interaction of LC3B Lys49 (green) with the phosphate group of FUNDC1 SEP17 (orange).
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