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The reaction mixture contained 12.5 μl SYBR green mix (Bio-Rad), 2 μl of mixed primers, and 2 μl cDNA template.
None of that, of course, bodes well for reducing skiers' carbon footprint, begging the question: Can luxury and green mix?
The expression level of E2-2 in different samples was tested with CYBR Green mix on the Rotor-Gene Corbett 65HO (Corbett Life Science).
Quantitative PCR was performed by using IQ Sybr Green mix (Bio-Rad).
The IQ Sybr Green mix (Bio-Rad) was used for the PCR reactions.
SYBR Green reactions were conducted with the IQ SYBR Green mix (Bio-Rad, Hercules, CA).
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QRT-PCR was performed using SensiMix Plus SYBR-Green Mix (No UNG).
The qPCR mixture contained 7.5 μL 2x Absolute QPCR SYBR Green mix (Thermo Scientific), 100 nM forward and reverse primers and 2.5 μL 100 times diluted cDNA.
Quantitative real-time PCR was performed using SYBR Green mix (TaKaRa Bio, Beijing, China) and the 7500 RT qPCR system (Applied Biosystems, Foster City, CA, United States).
Real-time PCR was performed using the SYBR Green mix (Biorad) with the MyIQ machine (Biorad).
PCR reactions were analysed using SYBR Green mix (Invitrogen).
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