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Expression of recombinant plasmid (pVAX-CDPK1) in vitro was analyzed by IFA at 48 h post-transfection, and specific green fluorescence was observed in Marc-145 cells transfected with pVAX-CDPK1, whereas no fluorescence was observed in the cells transfected with pVAX I (Additional file 1: Figure S1).
MARC-145, an African Green Monkey kidney cell line highly permissive to PRRSV infection, was used for PRRSV propagation and antiviral assays.
PRRSV susceptible cell lines, such as the African green monkey derived cell lines, like Marc-145, have the potential to overcome problems associated with the use of primary macrophages, such as up-scaling and safety.
In vitro, PRRSV only replicates in primary pig macrophages and African green monkey kidney derived cells, such as Marc-145.
In vitro, efficient PRRSV replication is only observed in primary pig macrophages (e.g. alveolar macrophages) [ 14], differentiated monocytes [ 15] or African green monkey kidney derived cells, such as Marc-145 [ 14, 16].
MARC-145 (MA-104 clone, African green monkey kidney cell line) cells were maintained in minimal essential medium (Hyclone) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 10 mM nonessential amino acids, and 100 U of gentamycin.
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MARC-145 cells are epithelial cells of the African green monkey kidney, and these cells are anticipated to produce only a minimal amount of cytokines, and their ability to produce IFN is limited unless they are stimulated.
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By William McKibben The New Yorker, September 17 , 1984P. 40 Talk story about Jean-Marc Roget, President of Chartreuse Diffusion, who is in charge of marketing Chartreuse, the green & yellow French liqueurs.
