Similar(60)
Assistant mayor for neighbourhoods, Green councillor Gus Hoyt, said he feared the story would make front page news as a "barmy council" tale.
To determine whether the phenotype is genetically linked to the PROP2 GUS construct, co-segregation analysis was performed using the F2 progeny from 23G13-2 crossed to WT. Results showed a segregation of 58 (green cotyledons and GUS positive): 16 (green cotyledon and GUS negative): 19 (reddish purple cotyledons and GUS positive): 4 (reddish purple cotyledons and GUS negative).
For β-glucuronidase activity in green tissues, RLK902::GUS dissected leaves were collected in microcentrifuge tubes on ice and incubated for 20 min in cold 90% acetone.
By Jonah Green and Marc Philippe Eskenazi September 12 , 2013Gus, the Central Park polar bear, died this summer, at the age of twenty-seven.
The resulting DNA fragment was sub-cloned into the pCAMBIA1304 vector containing a hygromycin phosphotransferase (hph) gene, a fusion of β-glucuronidase (GUS) and green fluorescent protein (GFP) gene under the control of the CaMV35S promoter.
In this line, we transiently expressed Myc-tagged AtSGT1a, Myc-E223K or β-glucuronidase (GUS) with green fluorescent protein (GFP -tagged PVX.
Annotated/predicted genes in A. indica were compared with a non-redundant database containing green plants gene identifier (gi) lists using homology-based searches.
To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA (β-glucuronidase, GUS) and gfp (green fluorescent protein, GFP), were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively.
Surprisingly, GUS activity in transgenic green fruit was considerably higher than leaves for all promoter constructs; however, in red, ripe fruit activities were much lower for the Cab19 promoter constructs than the mas35S::GUS.
Interestingly, transient expression of the RBCS3A construct showed no difference in expression of GUS between leaves and green fruit, implying that integration is necessary for specificity.
The other positions will be taken by Gus Hoyt for the Greens, Simon Cook for the Liberal Democrats and Conservative Geoff Gollop.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com