Exact(5)
After brief centrifugation, the supernatants were subjected to either immunoblotting with anti-V5 antibody or analysis of PAP1 activity using the malachite green colorimetric assay as described previously [50], [51], with minor modifications.
Serum albumin was measured using bromocresol green colorimetric method.
The PE and PC phospholipid fractions were estimated quantitatively by using the malachite green colorimetric method for phosphorous estimation [ 20].
These fusion proteins were purified and ATP and GTP hydrolysis activities were assayed for phosphate release using the malachite green colorimetric method for phosphate determination (Fig. 4) [ 41].
Albumin concentrations were determined in media collected from control and differentiation MSC cultures by Bromocresol green colorimetric assay (Biosystem, Barcelona, Spain) following manufacturer's recommendations and analyzed with spectrophotometer.
Similar(55)
The released inorganic phosphate (Pi) was assayed by a method previously described by Chan et al. [ 36] using malachite green as colorimetric reagent and KH2PO4 as standard.
Phosphate release was quantified by using a malachite green-based colorimetric assay for inorganic phosphate (Maehama et al., 2000).
The synthesized Mannich compounds were evaluated for their potential to suppress Hsp90 ATPase activity by the colorimetric Malachite green assay.
ATP hydrolytic activity of pig gastric microsomes was quantitated as orthophosphate release from substrate ATP using a colorimetric malachite green procedure [ 18].
An analysis of anonymized adult (> 16 years) samples with a range of CG levels from 15 to 22 g/l was undertaken for 50 samples at each level of CG (Abbott Diagnostics, Maidenhead, UK) using the colorimetric bromocresol green (BCG) method for albumin and the Architect Biuret method (16200 Abbott Analyzer; Abbott Diagnostics) for total protein (TP).
The thermo-dye dilution is performed by injecting simultaneously through the central venous catheter a cold indicator (cold saline) and a colorimetric indicator (indocyanine green).
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