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e H&E and f Congo red pathology slides reveal amyloid deposits with apple green birefringence under polarized light.
The present studies show that both B15D and B16D have characteristics of amyloidogenic proteins: the unbranched fibrils and ribbons stained with Congo red and displayed a green birefringence, exhibited a cross-β structure, and bound 1-anilino-8-naphthalenesulfonate.
Such fibrils did not bind Congo red and showed no green birefringence in polarized light.
These where strongly congophilic and showed bright green birefringence in polarized light.
Characteristically, red color indicates tightly packed collagen fibers whereas sites of tumor invasion exhibit green birefringence typical of increased extracellular matrix degradation.
Peptides No. 6, 11 and 12 all showed typical amyloid appearance after staining with Congo red with a strong green birefringence at polarization microscopy.
Similar(26)
The reference standard for diagnosing amyloidosis is histopathological confirmation of amyloid with Congo Red staining under cross-polarised light; with this staining, amyloid demonstrates apple-green birefringence [46].
Apple-green birefringence was examined by bright-field microscopy with a polarizing filter (Leica).
Amyloid histochemical analysis of DS lenses in the present study demonstrated Congo red staining (Fig. 3D) and intense apple-green birefringence (Fig. 3E) under cross-polarized illumination, findings consistent with the tinctorial requirements for designation as amyloid pathology [30], [31].
Ure2 forms amyloid-like filaments in vitro, which share several morphological, structural, and tinctorial features with amyloids, including enhanced resistance to proteolysis, increased Thioflavin T (ThT) fluorescence, and yellow-green birefringence in cross-polarized light upon Congo red binding [16] [19].
Amyloid deposits produce characteristic apple-green birefringence under polarized light when stained with Congo red.
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