Sentence examples for green assays from inspiring English sources
Cell viability and growth were assessed using Alamar blue™, lactate dehydrogenase and Pico green assays and morphology was studied by Toluidine blue staining and scanning electron microscopy.
ΔCt values were obtained by subtracting the average Ct values of triplicate SYBR green assays for 18s rRNA from that of the corresponding HLA-DR.
For all SYBR Green assays, standard curves were generated for each primer set to determine their efficiency, and dissociation curves were generated to detect non-specific amplification products and primer-dimers.
All transcripts were quantified in both human and monkey samples with either TaqMan or SYBR Green assays using TaqMan or SYBR Universal Master Mix Applied Biosystemss) with primer sequences listed in Table S1.
Wells for the SYBR® Green assays contained 12.5 µl of SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich), 0.25 µl ROX (Sigma Aldrich; diluted 10x), 3.5 5.5 mM MgCl2 (Sigma-Aldrich), 1 µl forward and reverse primer (10 mM), 5 µl of diluted cDNA and autoclaved MilliQ water to a volume of 25 µl.
Quantity was determined using standard Pico Green assays.
All of our DNA samples contained amplifiable DNA at amounts that correlated with Pico-green assays when tested by Alu-element Yd6; therefore, PCR inhibitors do not cause our negative results from the Guthrie cards.
However, the SYBR Green assay presents several disadvantages: SYBR Green I dye is an intercalating flurochrome that gives signal in proportion to DNA mass, not molecule number; SYBR Green assays rely on external standards that limit the absolute accuracy and are not universal to all sample types; finally, intercalating fluorochromes give signal from nonspecific PCR reaction products.
Each sample was tested in triplicate for SYBR Green assays, and in duplicate for Taq Man assays.
The qPCR assay was performed using SYBR Green assays (Applied Biosystems, USA).
The melting curves obtained after each PCR amplification confirmed the specificity of the SYBR Green assays.
