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In the second scenario, a traditional and an exposure-optimized deployment are designed for a throughput of 18 Mbps, corresponding to a receiver sensitivity of −82 dBm [ 17]. Figure 1(b) shows the two network deployments for scenario 2. The traditional network deployment consists of one 20 dBm green AP and the exposure-optimized deployment consists of the 4 low-EIRP purple APs.
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Green fluorescent Ap bacteria with normal morula morphology – that commonly seen with wild-type Ap – were clearly seen in cells.
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The traditional network deployment consists of the three 20 dBm APs (green) and the exposure-optimized deployment consists of the 17 low-EIRP APs (purple).
Although full-length GFP-FCHO1 or FCHO2 (green) concentrate at AP-2 positive surface spots, GFP-FCHO1 EFC + linker fully corrects AP-2 patterning while being diffusely associated with the plasma membrane in a featureless manner (C ) similar to the EFC domain alone (D ).
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To control for non-specific binding of the Cy3 secondary antibody or bleed through from the green (GFP) to red (anti-AP) channel, the AP antibody was left out, and, as expected, no signal was detected in the red channel (Fig. S2D-F).
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