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For SST the SST variations associated with the waters denser than 23.0 kg/m3 are superimposed for the SMOS (blue), NCEP (red) and CSIRO (green) analysis.
These sensor-based technologies, owing to their function and to the elements involved in their design, potentially generate fast, selective, sensitive, safe and green analysis solutions.
SYBR Green analysis was performed on a Lightcycler (Roche).
ALU J and TLR2 data was compared with pico green analysis using the Spearman rank test.
Typical reactions included 250 μM polyP and 20 units/mL CIAP; the liberated monophosphate was quantified using malachite green analysis.
UCP2 sequence-specific FAM probes (Applied Biosystems, Foster City, CA, USA) and primers (SYBR Green analysis) were used in independent QPCR assays.
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qRT-PCR (SYBR-green) analysis was performed on an Applied Biosystems 7500 Real-time PCR system.
A RAP-Green analysis was carried out using the original tree from PhyML to predict duplications [ 58].
A RAP-Green analysis was performed from the initial PhyML tree to improve gene function inferences and predict gene duplications in phylogenetic trees [ 90].
Our assay therefore combines the convenience of SYBR Green based analysis with the specificity of probe based analysis and is applicable to all models of Cre-lox mediated conditional gene deletion.
Comparing the green (HCD analysis searching two ion types) and the red (UVPD analysis searching nine ion types) analyses results in a 2.8-fold increase (from 1.2 to 3.3%) in false positive rate at the highest (least confident) E values examined.
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