Exact(3)
SYBR Green analyses also included a dissociation protocol.
SYBR Green analyses were followed by dissociation curves in a temperature range of 60°C~90°C to assess the amplification specificity.
PCR amplicons were previously evaluated on agarose gel and, during SYBR green analyses, primer dissociation curves were checked in each run to ensure primer specificity on human and zebrafish mRNA.
Similar(57)
Proteins in the 305 and 80 sets, as well as 37 proteins with limited conservation within Dictyostelia (green) were analysed with Phobius63 for transmembrane domains and signal peptides (Supplementary Data 4).
The green group analysed civil aviation authority figures, which show business trips by air are down 13% since 2000, and as much as 23% at Heathrow, where the aviation industry has argued for a third runway despite Labour, the Conservatives and Liberal Democrats all opposing such an expansion.
A region of green colour analysed from JK01 was found to be relatively enriched in copper.
JC-1 was excited at 488 nm and the monomer signal (green) was analysed at 525 nm (FL1).
For detailed description of population species see Additional file 2. Of the 208 total green turtles analysed (FP exhibiting and clinically healthy), 61 (29%) were PCR positive for CFPHV.
In its annual "green budget", which analyses the state of the public finances before Osborne's March budget, it said a weaker-than-expected growth performance or over-optimistic forecasts for tax receipts might force the Treasury to make deeper cuts in welfare, cut spending further or raise taxes.
A migration rate of 0.0001 (per gene per generation) always led to the worst success rate, and failed to reach a success rate of 1.0 even when all 1,000 loci were used in analyses (green circles, Fig. 4b d; Supplementary Tables S3 S5).
Evidence that a 10.4% rise in house prices last year – three times the rate of inflation – had sparked feverish demand for homes, especially in London and the south-east, was dismissed by the IFS in its annual "green budget", which analyses the state of the economy and public finances.
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