Glycogen phosphorylase a (from rabbit muscle), glycogen, glucose-1-phosphate, malachite green, and ammonium molybdate were purchased from the Sigma Aldrich Corporation.
After incubation, 100 μL of the phosphate detection reagent (2.6 mM Malachite Green, 1.5% ammonium molybdate, and 0.2% Tween 20) was added to each sample.
After neutralization with HCl, a mixture of malachite green and acidified ammonium molybdate was added to form a complex of phosphomolybdate and malachite green.
This work presents an experimental study focusing on the preparation and characterization of modified green clay with quaternary ammonium salts alkyl dimethyl benzyl ammonium chloride (ADMBAC) and distearyl dimethyl ammonium chloride (DSDMAC) intended to be used as adsorbent in the process of removing oil emulsion in an oil water system using finite bath.
Nitrogen/sulfur dual-doped graphene with uniformly coupled N doped Fe2O3 (N-Fe2O3/NSG) composite has been successfully synthesized via an easily one-step green method, in which ammonium thiocyanate (NH4SCN) is applied as nitrogen source and sulfur source without adding other alkali sources or dispersants.
Q61E shows the opposite effect with decreased blue and increased green emission at high ammonium concentrations.
Enzymatic activity was stopped by addition (45 μl/well) of colorimetric reagent (0.07% malachite green, 3.7 NH2.27, 2.27% ammonium molybdate tetrahydrate, 0.134% Tween-20).
The resulting phosphate was quantified by a dye solution containing 0.033% w/v Malachite Green, 1.3% w/v ammonium molybdate and 1.0 M HCl.
The ammonium molybdate/malachite green technique of P i quantification is extremely sensitive, capable of reliably detecting P i at concentrations as low as 100 pM [ 22].
As a case study, protoplasts of transgenic cell lines stably transformed with an ammonium transporter-green fluorescent protein (PutAMT1 1-GFP) fusion were successfully used for subcellular localization analyses in PutAMT1 1-GFP.
When P i is complexed with ammonium molybdate and malachite green in 4 M HCl, it creates a green color which can be quantified by reading the absorbance spectrophotometrically at 660 nm in a FLUOstar Omega (BMG Labtech, Cary, NC) and compared to a standard curve of known P i concentrations [ 22].
