The scarcity of and, most importantly, the highly heterogeneous nature of normal human bronchial epithelial (NHBE) cells with regard to their ACE2 expression and, thus, permissiveness to SARS-CoV infection [39], [40] (Tseng, C.K. et al., unpublished observation) greatly limits their use in exploring the genome-wide responses to viral infections.
The B40 Sep-OTS was prepared as described previously except with 5 tandem copies of tRNASep, which greatly enhances OTS activity (unpublished).
This greatly increases the chances of pregnancy (unpublished data).
Fortunately, techniques for resuscitation have advanced and survival after out-of-hospital cardiac arrest has improved greatly in the past years (New York City Fire Department, unpublished data).
First, the use of restriction enzymes with 6-bp recognition sites is not recommended for cDNA pools [[ 6], Kivioja, unpublished data], as it greatly reduces the number of fragments generated per PCR reaction.
In contrast to Mitf-M, tyrosinase expression is not generally maintained (MST-2 cells are an exception although even in this case, tyrosinase levels are greatly reduced following prolonged culture (KAWL, unpublished observations)).
In the decades since, her relatively small body of work some hundred published poems, a dozen stories has been greatly outweighed by volumes of letters, previously unpublished poems and drafts of poems, biography, and criticism.
However, phospholipid synthesis was not greatly altered in these stalled cells (T.K.S. unpublished data), allowing serine uptake (at about 20% normal levels) and incorporation into phospholipids to continue.
Her switch in policy will more closely align the Scottish Tories with proposals for greater Scottish "home rule" coming from the Liberal Democrats and as-yet-unpublished plans from Labour to greatly increase devolution.
For example, in a study we found that, besides culturally adapting the materials used, personal contact in the form of telephone reminders instead of written reminders greatly enhanced participation (I. G. M. van Valkengoed, K. Stronks, unpublished results).
