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Besides, the concentration of 10-8 M provided the greatest induction at every time point except at 48 h, which suggests that the optimum concentration of E2 for the cells is 10-8 M. Therefore, we treated the cells with E2 (10-8 M) to seek the time point of the greatest induction of SBD-1 expression.
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Having demonstrated that the greatest induction was observed at 3.5 h in E2-induced up-regulation of SBD-1 expression, we went on to elucidate the pathway by which signal was transduced to the nucleus.
As is evident from the figure, the induction of SBD-1 mRNA expression was very rapid after the cells had been treated for 2 h and the greatest induction was observed at 6 h, which suggests that the highest expression levels were around 6 h.
Figure 1E shows that the greatest induction of SBD-1 expression was observed at 3.5 h and the induction of SBD-1 expression has significant differences from 1.5 to 10.5 h.
Genes related to jasmonic acid (JA) formation, for example, are already constitutively expressed at high levels and exhibit greater induction in S-oaks than in T-oaks.
Similarly, for randomisation 2, final height was significantly greater with pubertal induction at 14 years than at 12 years (3.8 cm, 0.0 to 7.5; P=0.05).
Interestingly, increased expression of dSir2 in the dSir2EP2300 line extends life span more reliably and to a greater extent than induction at similar levels from the UAS-dSir2 transgenes.
A small subset of the Early Genes shows a variant pattern of gene expression in which the transcripts are up-regulated from the first 5 minutes of blood uptake through 1 hour PBM followed by a repression in expression from 3 to 24 hours PBM and then a greater than twofold induction at 48 hours PBM.
Three probe sets representing transcription factors also exhibited a greater than 10-fold induction at these later time points.
As shown in Figure 2A (left and right panels), exposure of cells to hypoxia determined a HIF-1α protein induction, at a greater extent in bcl-2 transfecomparedompared to control cells, as previously reported.
However, when caring for a pregnant woman at term, a clinician's options are not between induction of labor and spontaneous labor; rather, the options at hand are induction of labor versus expectant management of the pregnancy, the latter of which results in either spontaneous labor or induction at a greater gestational age.
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