For example, we have confirmed that NV31252, which was annotated as a protamine-like gene, (i) has one of the greatest arginine contents of any gene in the N. vitripennis genome, (ii) is uniquely expressed in the wasp testis, and (iii) exhibits the greatest expression level of those genes that are uniquely expressed in this tissue.
Cyp26b1, which shows the greatest expression level change, also expands anteriorly, with anomalous increased expression in r2, r3 and r4.
Three lines expressing Gal4 were used: ptc-Gal4, which expresses in the A compartment with greatest expression levels in a stripe along the compartment border; en-Gal4, which expresses in the P compartment; and C765-Gal4, which expresses in most wing disc cells.
With the exception of OsSRP-FRS, the identity of the highly expressed genes matched closely to those with greatest expression levels reported in the Rice GE: Gene Expression Analysis microarray data.
The moricin subfamily, which shows the greatest expression levels in the malpighian tubules, showed the greatest upregulation at 24 hpi; the expression level was increased more than eight-fold.
Of interest, 60% of the genes with the greatest expression levels on Avicel are annotated as Pth11-related.
As the CaBPs exhibit greatest expression levels in neuronal tissues we reasoned that these cells would provide a more accurate reflection of correct sub-cellular localisation.
Vertebrates possess four MEF2 family member genes (-A, -B, -C, -D), which demonstrate complex overlapping spatial and temporal expression patterns in embryonic and adult tissues, with greatest expression levels seen in striated muscle and brain (Potthoff and Olson 2007).
Temporally, the greatest expression levels of Cyp6d2 occur during the larval and prepupal stages (Chintapalli et al. 2007), which coincide with the days immediately after treatment in our sensitivity assays.
Finally, the choline dehydrogenase gene CG9505, which shows evidence for an adaptive increase in expression in populations outside of sub-Saharan Africa (Saminadin-Peter et al. 2012; Glaser-Schmitt et al. 2013), has its greatest expression levels in the Malpighian tubules (Chintapalli et al. 2007).
Furthermore, we have shown that, while both IL-1 and TNF are expressed in IVD and upregulated with degeneration, degenerate IVDs show a much greater expression level of IL-1β than TNF-α and that, while the IL-1R1 was upregulated in degeneration, the TNF receptor 1 (TNF-R1) was not [ 8, 13, 14].
