One of the greatest difficulties encountered in identifying genomic signatures of selection is in distinguishing changes that have occurred due to demographic as opposed to selective forces [ 9].
I pointed out the great difficulties encountered by the German minorities and the undignified sititation, as I should like to put it, that is, the always undignified difficulties confronting Germans who wanted to travel to East Prussia.
This underlines the great difficulties encountered in reconstructing active GPCRs in model membranes.
While this is all very encouraging, this information avalanche often proves daunting to biologists for there are great difficulties encountered in searching, retrieving, interpreting or managing the data.
Despite some variability, data generally demonstrate a positive trend, showing improvements for most indicators with fairly frequent drops in both second semesters, which most probably reflect the greater difficulties encountered during the summer holiday season, when personnel is reduced, thus affecting the provision of services.
If anything, the greatest difficulty we encountered in 2012 was trying to get over the year before, especially the stoned and slurry post-chopped-and-screwed sound of Clams Casino-style hip-hop and the extraordinary breeding ground for diverse new talent that was Odd Future.
During the course of the study, the greatest difficulty was encountered in the questionnaire implementation stage due to the timing of the academic recess for students in Colombia, which coincided with the data collection date.
The greatest difficulty was encountered with the uranium slugs produced by Mallinckrodt and Metal Hydrides.
One worker recently told me that the greatest difficulty was encountered in the final stages of healing of the soft tissues.
To the surprise of many, possibly including himself, the greatest difficulties he encountered were not in managing the coalition with the Lib Dems but with keeping control of the increasingly vocal and rebellious right-wing of his own party.
