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Codon 12 mutations had slightly greater transforming potential than codon 13 mutations and consistently greater transforming potential than the codon 61 Q61H mutation.
In particular, in vitro and in vivo studies indicate that KRAS codon 12 mutations have greater transforming capacity when compared with codon 13 mutations [ 23, 51, 52].
CrkI regulation must be different because it lacks the regulatory tyrosine, a characteristic that was long ago presumed to be associated with its greater transforming activity [ 37].
It has been suggested that FOXM1b is present in the predominant species of cancer cells and has a greater transforming potential compared with FOXM1c [3].
Although experimental evidence has suggested that mutations in KRAS codon 12, particularly p.G12V, confer lower GTPase activity (Bollag and McCormick, 1995; Al-Mulla et al, 1999), which may translate to greater transforming potential, our data are not consistent with a clear difference in the prognostic significance of somatic KRAS mutations by codon.
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From our model, we would predict a change in E-cadherin levels for a β-catenin activating mutation to have the greatest transforming properties.
Mouse 3T3 cells expressing T1977I, L2209V, or S2215Y mutants of mTOR (which are among those with the greatest transforming activity) were subjected to a focus formation assay in the absence or presence of 100 pM rapamycin.
Previous studies have indicated that FGF8b is the predominant form in breast tissue (Marsh et al, 1999) and that FGF8b has been shown to have the greatest transforming ability (MacArthur et al, 1995b).
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