Some of these might reflect an inherent greater sensitivity of the microarray technique used for detection of transcript changes, than that of the mass spectrometry method used to probe changes in protein levels.
These differences may be explained by the greater sensitivity of the nested RT-PCR used in those studies.
The fact that we detected reductions in GBA messenger RNA levels in cells bearing the L444P mutation probably reflects the greater sensitivity of the quantitative PCR technique we used, compared with the northern blot techniques used in earlier studies.
The greater sensitivity of HRM allowed longer amplicons to be used (534 base pairs was successfully used for the region surrounding the 6174delT mutation) which allows for the screening of unknown mutations as we have demonstrated.
Due to the greater sensitivity of the rosette inhibition assay, this was used in subsequent experiments.
The inconsistency between our results and those of Woodman et al. might be a result of the greater sensitivity of the continuous-report measure that we used here, relative to change detection tasks.
It would also require greater sensitivity of response which may be achieved by the use of microelectrodes and this is a route that should be investigated further.
To confirm the greater sensitivity of the cobas EGFR test compared to Sanger, a third comparator method was used, MPP.
Studies that used the BAI obtained higher effect sizes, maybe due to a greater sensitivity of this instrument.
This reflects the greater sensitivity of array CGH.
