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The data in Figure 2A and 2B follow a similar trend, in which there are greater fold changes in gene expression following dnFGF4 injection than with dnFGFR1.
As anticipated, quantitative results of qRT-PCR were more sensitive in general with greater fold changes than that of microarray.
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Only two immune response genes (complement receptor 1, AB109024 and NALP1, XM_415289) had a significantly greater fold change in expression in AN than BN (Table 1).
Final lists of significantly changed genes used in class comparisons were filtered to include only those transcripts that showed a 1.25-fold or greater fold change in expression level relative to the control group.
Only 46 proteins demonstrated a 3 or greater fold change in either of the two cell types.
Additionally, lower abundance marks require greater fold change to be deemed significant and, conversely, higher abundance marks require lower fold change to be deemed significant.
The interest statistic reflects a biologist's understanding that a gene with a greater fold change (in absolute value) than other genes is potentially more interesting.
The qPCR results show a similar trend of up-regulation of DHRS7C gene in polled versus horned and scurs animals, but with much greater fold change.
The data showed that qPCR was more sensitive and showed greater fold change differences compared to the microarray analysis, which was in keeping with previous reports [ 26].
For starvation stress, only genes with a twofold or greater fold change were included in the GO analysis to reduce the dataset to a manageable number.
Any gene with two or greater fold change in the fluorescent population was annotated as sexually committed whereas any gene with 0.5 or less fold change was annotated as asexually committed.
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