Greater copy numbers were calculated as ratios relative to a single copy value.
This could, in part, explain the much greater copy number in mammals than in teleosts.
Identification of additional animals infected with a greater copy number of the novel haemoplasma would be required to enable further characterisation and complete molecular description.
Of the clusters, five contained genes with higher copy numbers in SN15, and three clusters contained genes with greater copy numbers in Sn4.
This discrepancy in signal strength, particularly for the universal centromere probe PviCentC, suggests that a greater copy number of this repeat is present in Chromosome 3 of one subgenome than in all other chromosomes.
Our previous report demonstrated that well-differentiated PCa cells (LNCaP) had the greater copy number of mtDNA, the higher oxygen consumption, but Ras expression and activation were reduced as compared with those in poorly differentiated PCa cells (PC-3).
Southern hybridisation using the E2 and E6 probes confirmed the presence of greater copy numbers of E2 than E6 in CaSki, and 1-dimensional analysis of the autoradiogram gave an E2/E6 ratio of approximately 2. The data from the detailed cell line analysis confirms that errors in SYBR green qPCR-based HPV16 gene quantification should be allowed for, especially at low copy number.
Surprisingly, these results showed that, contrary to what had been observed during our former comparisons [ 26], most genomic differences within the citrus strains are not associated with deleted ORFs, but with elements that are present in greater copy number in the tested strains, when compared to Xf strain 9a5c (see Table 2, and Additional File 1).
For the visualization of comparative profiles from each analyzed strain, we applied the method proposed by Smoot and coworkers [ 71], where ORFs shared by the reference and each test strain were labeled 0, while ORFs exclusive to the reference strain, or present in greater copy number in the test over the reference strain were labeled 1 and -1, respectively.
Spots that showed a Reference/Test ratio < 1 2 were considered to be present in greater copy number in the test over the reference strain, as proposed by Smoot and coworkers [ 71], while spots that showed an average Reference/Test ratio > 5 1 were considered to be missing in the test strain.
