REG-FFA medium, which does not contain glycerol, supported the greatest cell mass accumulation (6.05 g/L).
The yield would also be enhanced further by the greater wet cell mass obtained under this condition.
However, for ET149 a change of inducer concentration to 50 ng/ml gave only slightly improved yields (6.3 µg vs. 6.15 µg), demonstrating the compensating effect of greater wet cell mass at 20 ng/ml inducer concentration.
The morphology was markedly altered, with a different cell shape, and a greater dead cell mass was clearly observed in the supernatant after 4 h (Fig. 3C) of the cells being exposed to heat.
Thus, we concluded that B 9-23/IFA immunisation did not affect beta cell replication, but instead modulated the autoreactive immune response, an observation that also explains why this regimen is not effective at more advanced stages of the disease, when greater beta cell mass is lost.
The ratio of microbial cell carbon to organic acid carbon tended to be greater for PHL (0.90) and INU (0.86) than for GLC (0.69), indicating a greater yield of cell mass per amount of substrate fermented with fructans.
However, in the pre-fermentations with yeast that was cultivated with an inhibitor concentration that corresponded to 10 % WIS, the variance in xylose utilization increased significantly, correlating with greater variance in cell mass yield, viability, and transcriptional changes with increasing inhibitor concentrations in the feed.
18– 21 Islet histology in mice treated with DPP-4 inhibitors followed by streptozotocin revealed greater β-cell mass suggesting improved β-cell survival.
In that study, we reported an ∼50% greater β-cell mass (0.8 to 1.2 g) in obese humans compared with lean humans (2).
We speculated that the difference between greater β-cell mass and improved function could be determined by observing the duration of any treatment effect; i.e., if the intervention resulted in increased β-cell mass, improved pancreatic insulin production would be expected to persist beyond the exenatide treatment.
