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The hZwint-1 non-interacting mutant N1 has been previously shown to be dynamic at prometaphase kinetochores compared with stable wild type hZW10.[17] Because N1 is more dynamic at prometaphase we expected it would have faster and greater accumulation at spindle poles following NDGA treatment, however, we actually observed reduced spindle pole accumulation.
Western analysis detected increased levels of VSG after four hours in lysates from cells treated with 10µg/ml MG-132 (data not shown), while the effect was substantially more pronounced with 20µg/ml MG-132, where VSG levels were clearly increased after only two hours and with greater accumulation at four hours (Figure 2).
We used a non-ortholog based strategy and identified four genes that exhibited increased mRNA expression as development proceeded from stage 14 to stage 17, a set of genes that responded to E2 exposure, and two genes (MALAT1 and C16ORF62) that show greater accumulation at the male producing temperature than at the female producing temperature.
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The metabolic patterns of change showed that coumarate, the immediate precursor of phenylpropanoid, had greatest accumulation at pre-veraison stage declining afterwards in both cultivars; it is likely incorporated into downstream metabolism.
While it has been demonstrated that PEGylation of NPs causes a greater accumulation of drug at the tumor site by passive targeting, active targeting of the NPs can aid in selection of the target cell type within the tumor site and internalization of the NPs to a greater extent inside the target cells.
It has been shown that highly nucleosome occupancy downstream of polyA sites strongly correlated with a more favorable mRNA structure and greater accumulation of RNAP II at the polyA site [ 21], and improved the usage frequency of polyA sites.
In liver, greater accumulation of ANG located at the endothelial layer of hepatic sinusoidal blood vessels compared to hepatocytes suggest hepatocytes as potential sources of increased synthesis of ANG after SFG rickettsial infection, but endothelial cells are the targets of upregulated ANG.
However, under salt and drought stresses, WT plants showed a greater accumulation of H2O2 than transgenic lines at all time points.
This coincided with a greater accumulation of GABA (neurotransmitter and an insect antifeedant) at the feeding site.
Dysferlin is found at all stages of disease with greater accumulation in more advanced disease (CDR 3).
At 3 hours, with similar lung bacillary loads, HN878 infection caused greater accumulation of mononuclear and polymorphonuclear leukocytes (PMN) in the lungs, compared to animals infected with CDC1551.
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