Increasing the lysine and histidine residues reduced particle size and increased zeta potential of DNA complexes, leading to greater gene expression efficiency.
There are limits to the improvement in adaptation when sexual selection is strong and favours greater gene expression in males (the heterogametic sex).
Particularly, the largest QD bundled NPs examined, QD655, had a much higher uptake capability and greater gene expression ability than the other QD NPs (QD525, QD565, and QD605).
VX2 tumors infected with Ad-CMV-HA-SSTR2 upon intra-arterial delivery with AII had greater in vivo biodistribution, thus greater gene expression, than those without AII (p < 0.01, n = 6).
Similarly, VX2 tumors infected with Ad-CMV-HA-SSTR2 upon IA delivery with AII had greater biodistribution, thus greater gene expression, than those with the negative control Ad-CMV-GFP (p < 0.01).
VX2 tumors infected with Ad-CMV-HA-SSTR2 upon IA delivery had greater biodistribution, thus greater gene expression, than those with the negative control Ad-CMV-GFP (p < 0.02).
In this analysis, LPS stimulation of DC induced the greatest gene expression changes in principle component (PC 1 and PC2.
We hypothesize that such secondary metabolite pathways may have higher levels of gene duplication and greater gene expression diversity than primary metabolite enzymes.
We therefore used principle component analysis (PCA) of transcriptional profiles to compare components of the data that are responsible for the greatest gene expression differences (Figure 6B).
Some support for this comes from the observation that, among laboratory strains, greater gene expression variation is observed among males than among females [42].
