Sentence examples for gravity column from inspiring English sources

The urea crude fractions were subjected to protein purification using Ni-NTA Agarose gravity column (Qiagen, Germany) at denaturing conditions.

The supernatant was loaded onto a gravity column of Ni2+ affinity resin (Ni-NTA, Qiagen).

The targeted spot was isolated using gravity column chromatography and a white coarse solid (2) was obtained.

The slurry was loaded onto a 15-mL gravity column (Bio-Rad) and washed with a buffer containing 20 mmol/L Hepes, pH 7.5, 50 mmol/L NaCl, and 10% v/v glycerol for 20 30 column volumes.

Cells were harvested by centrifugation, lysed by sonication, and clarified by centrifugation; the supernatant was then applied to a nickel-nitrilotriacetic acid (Ni-NTA) resin gravity column (Qiagen, Valencia, CA, USA) that had been previously equilibrated with PBS (137 mmol/L NaCl, 2.7 mmol/L KCl, 50 mmol/L Na2HPO4, and 10 mmol/L KH2PO4 at pH 7.4).

The structural design of 7 World Trade Center therefore included a system of gravity column transfer trusses and girders, located between floors 5 and 7, to transfer loads to the smaller foundation.

Two cases are considered: gravity columns perfectly fixed, and gravity columns pinned at the base.

Furthermore, gravity columns bending moment demands are distributed between braced frame and gravity columns in proportion of their relative flexural stiffness.

A simplified theoretical formulation is presented to characterize the effect of gravity columns.

Three different approaches are proposed for the design of the braced frame and gravity columns.

This would be the case when horizontal struts are used to connect gravity columns to braced frames at every tier level, as is often seen along exterior walls.