Exact(8)
Furthermore, grass anchor probes that locate on LG 3 of ryegrass [ 11] were selected.
PCR primers were also designed from the grass anchor probes developed by Van Deynze et al. [ 36].
Additional file 1: Summary of EST clones selected from rice chromosome 1 and grass anchor probes, and results of the SSCP analysis.
Similarly, two primer pairs (28.6%) derived from grass anchor probes successfully amplified clear PCR products from the female and/or male parent; both were polymorphic between the parents in SSCP analysis.
On that map, the closest grass anchor probe, CDO460, linked tightly to the QTL of Curley et al. [ 11] but is genetically over 25 cM distant from SSR markers, 08-08B and 09-12H, both of which are closely linked to LmPi2 on our map of LG 3.
Unfortunately, we could not directly distinguish between that QTL and LmPi2 locus since most grass anchor probe-derived markers, some of which are located around the QTL of Curley et al. [ 11], could not be added to our map of LG 3 because of unsuccessful PCR amplification in this study (Additional file 1).
Similar(52)
Without native grasses to anchor the topsoil, fields crumbled to dust.
It is effective in soft bottoms, and somewhat better in grass than other lightweight anchors.
"Boy oh Boy" the anchor gushes, "That grass fire sure is smoky!" No reporter on the scene, No live van, No editing, No play back machines No producers.
The integration of RFLP markers previously mapped in other grass species has provided the anchor points to align the pearl millet linkage groups to other cereal genetic maps.
To examine if assembled grass genomes would serve to anchor other RAD markers, we aligned polymorphic sequences to the 430 Mb Oryza sativa and 300 Mb Brachypodium distachyon genomes using a modified CIP/CALC method [ 24- 26].
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