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The potential anti-irritant substances including β-sitosterol, Magnoliae flos, β-glycyrrhetinic acid, SC-glucan, Ginko extract, Raspberry extract, Schisandra extract, Cola extract, Enna complex or Vegetol red grapevine extract were evaluated for their inhibitory effects on retinol-induced cytokine (MCP-1 and IL-8) secretion in vitro cultured human fibroblasts.
The radioactivity of each grapevine extract eluate was determined using a Canberra Packard TriCarb 2100TR (Canberra-Packard, USA) liquid scintillation counter, each sample was counted over 5 min with the average recorded.
Kim and colleagues (2003) have demonstrated that concomitant application of natural agents or extracts like β-sitosterol, Magnoliae flos, β-glycyrrhetinic acid, Scleroglucan, Gingko extract, Raspberry extract, Schisandra extract, Cola extract, Enna complex, or Vegetol red grapevine extract could also be useful in counteracting the irritant effects produced by topical retinoids.
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Multiplex RT-PCR conditions were established for the simultaneous detection in grapevine extracts of either arabis mosaic virus, rupestris stem pitting associated virus and malate dehydrogenase mRNA, or grapevine virus A, grapevine virus B, grapevine leafroll associated virus-3, and RubiscoL mRNA.
Caudalie, a French company, touts a product line that is "high in polyphenols, resveratrol, and viniferine," an extract of grapevine stalks (though not the sort of extract whose daily intake in high doses may give you a purple nose).
Total RNA from grapevine tissues was extracted according to the methods described in [ 59].
Total RNA from different grapevine leaves was extracted according to the methods described by Reid et al.[ 30].
Comparisons were also made between the conventional one step RT-PCR and TaqMan® RT-PCR for the detection of these viruses using four-fold serial dilutions of both purified RNA and crude extract prepared from grapevine tissue.
The grapevine plant extracts were screened for radical scavenging capacity of individual phenolic compounds, using an on-line HPLC post-column reaction assay [ 36].
Figure 8 shows COADS chromatograms corresponding to the phenolic compounds separated in a methanolic extract of thiamine-treated grapevine plants and their corresponding antioxidant activities.
In this study, we present an optimized VIC method to extract soluble proteins from AF of grapevine leaves and a well-defined interactive map of these proteins.
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