Sentence examples for granules was performed using from inspiring English sources

SEM examination of starch granules was performed using a Hitachi Model S-4700 scanning electron microscope at 10.0 kV.

Microscopy to determine the circularity of granules was performed using an Andor spinning disk confocal microscope as described above with a UPlanSApo 100 × 1.4 oil objective.

Immunofluorescence analysis of cortical granules was performed using lens culinaris agglutinin as described previously (Connors et al., 1998) except that the ZPs were left intact to allow visualization of perivitelline space material.

Measurement of the circularity of P bodies and stress granules was performed using a custom-made Matlab routine (see Supplementary file 2) from maximum intensity projections, according to the following equation: C i r c u l a r i t y = 4 π A r e a (P e r i m e t e r ) 2, where the value of circularity is 1 for a perfect circle and the value decreases as it deviates from the circular shape.

Also, morphological observations of both granules were performed using scanning electron microscopy under an accelerating voltage of 15 kV (S-3400N, Hitachi High-Technologies, Tokyo, Japan).

Tracking analysis of secretory granules in the images was performed using the manual tracking plug-in (Fabrice Cordelieres, Orsay, France) and the chemotaxis and migration tool (Ibidi) of ImageJ 1.43u (National Institutes of Health, Bethesda, MD, USA) as described in the μ-Slide Chemotaxis protocol.

The whole process of the dot-blotting was performed using Dig Easy Hyb Granules, Dig-Wash and Block Buffer Set, Anti-Digoxigenin-AP and NBT/BCIP ready-to-use tablets (Roche) following the manufacturers' instructions.

Wet granulation experiments were performed using the 3D printed designs to test the hypothesis that the correlation between the granule shape and maximum granule size and the screw element geometry is predictable a priori.

Aggregation, dense granule secretion and spreading assays were performed using washed platelets.

Fibrinogen binding, α-granule secretion and calcium mobilization assays were performed using platelet-rich plasma and whole blood was used in impedance aggregometry and thrombus formation experiments.

Our experiments were performed using standard molecular biology procedures to measure the intensities of positive staining and positive rates of the samples by immunohistochemistry: Brown granules in cell nuclei or cytoplasm are considered as positive signals.