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These parameters were correlated to granule size distribution, mean granule size and granule shape.
It was found that agglomeration mechanism involved, i.e., granule shape, can be greatly influenced by binder properties.
Wet granulation experiments were performed using the 3D printed designs to test the hypothesis that the correlation between the granule shape and maximum granule size and the screw element geometry is predictable a priori.
Characteristics affecting starch digestion are the amylose/amylopectin ratio, proportion A-/B-starch granules, starch granule shape, crystallinity, lipid content, nature of the protein matrix surrounding starch granules and the overall architecture of the starch granules.
It was shown that there is a qualitative relation between the sedimentation behaviour (as measured by the sediment ratio) and the granule shape (solid or hollow) and a quantitative relation between the thickness of the shell (for hollow granule) and the state of dispersion and the nature of the binder used.
The differences in the starch granule shape and the arrangement of the granules resulted in the higher PGWC and DEC percentage in P02428, which was in agreement with previous research (Guo et al. 2011; Li et al. 2014b).
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The quadruple deletion strain, Re2005 (ΔphaZ1 ΔphaZ2 ΔphaZ3 ΔphaZ5) exhibits a slight decrease in the area ratio, suggesting other PhaZ enzymes may also have granule shaping and remodeling functions.
Furthermore, silique chloroplast appeared in oval shape and with relatively small proportion of matured starch granule (Figure 3A).
The flexibility in process settings such as the binder addition method (wet vs. dry), screw configuration, screw speed and material throughput allows to modify the granule size and shape.
Binder particle size had the most pronounced influence on granule size and shape, suggesting the importance of this parameter in achieving desired granule properties.
A D-optimal design (29 experiments) was used to assess the influence of process (screw speed, throughput, barrel temperature and screw design) and formulation parameters (starch content of the filler) on the process (torque), granule (size distribution, shape, friability, density) and tablet (hardness, friability and dissolution) critical quality attributes.
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