Conceivably, the observed reduction of M6PRBP1 upon DFMO treatment could thus impair M6PR-mediated targeting of granule compounds, providing a partial explanation for the effects seen on granule storage/morphology.
Hence, serglycin holds a key role in the assembly of granule dense core material and this has proven vital for proper storage of a number of secretory granule compounds [11], [12], [14].
Further, DFMO treatment did not lead to a reduction in the storage of either MC-CPA or mMCP-6, granule compounds that were previously shown to be strongly dependent on serglycin for storage within the granules [11].
At this stage we cannot explain the mechanisms behind this observation, although we may hypothesize that the reduction in other granule compounds may facilitate the accumulation of β-hexosaminidase.
Since this protein plays a clear role in secretory granule formation, in particular as a component of the molecular machinery that sorts many granule compounds to the actual granules, its alteration could therefore provide the best direction, as compared to the other proteins detected, to further explore the mechanisms underlying the observed DFMO-induced effects on granule storage/morphology.
However, the incorporation of molecules containing humic substances into the granules of compound NPK fertilizers is not a common practice mainly due to technical difficulties and problems associated with the manufacture process of these mineral fertilizers.
The TCM granules were compound preparations of TCM and its components are shown in Table 2.
In the compound granules, the individual starch granules were found loosely attached to its neighbouring granules and most of the compound granules were also loosely attached to its neighbouring compound granules.
Different sized starch granules were often observed in the same compound granules (Fig. 3h).
Zengmian Yiliu granule (ZMYL), a compound preparation of traditional Chinese medicines, has been used in clinic as a complementary and alternative medicine for attenuating CDDP-induced toxicities and enhancing the tumor therapeutic effect of CDDP.
