Here we describe a rapid technique for skin granular gland transcriptome cloning from a surrogate tissue the secretion itself.
Amphibian skin granular gland secretions are consistently recognized as rich sources of multiple biologically active compounds [1], such as antimicrobial peptides (AMPs) [2], bradykinins, biogenic amines, complex alkaloids, etc.
In the resulting bitransgenic (BT) offspring (pTight-Ets1/ INV-tTA), HA-Ets1 can be induced in the upper spinous and granular layers of skin and other stratified squamous epithelial tissues when doxycyline (Dox) is withheld.
Finally, two subjects were documented to present with mandibular multiple giant cell lesions (MGCLs), which were associated with multiple tumors, including abdominal rhabdomyosarcoma, cerebral glioma, and skin granular cell tumors in one subject.
The terminal differentiation marker, filaggrin, which is normally expressed in the epidermal granular layer of WT skin, was detected in multiple layers in RASSF9−/− mice skin (Figure 10).
Granular cell tumour of skin is an uncommon benign neoplasm of neural origin.
In the past decade, transcriptome data of the granular glands of anuran skin has given new impetus to investigations of the putative constituent peptides.
SRC3 was detected in the suprabasal and granular layer of the skin, similar to cathelicidin expression.
As shown in Fig. 6, both FLG2 and FLG were mainly expressed in the granular layers of human skin.
Growth suppressor genes are activated by C/EBPδ in primary keratinocytes: EDG-8 [75], STAT2, a TF expressed in the granular layer of the skin [76], E2F2 and IRF1.
