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C57BL/6 male mice aged 6 to 8 weeks and weighing 20 to 22 grams were obtained from Nanjing University Model Animal Research Center and used for this study.
Green grams were obtained from the supermarket.
Male Lewis rats weighing approximately 300 grams were obtained from Charles River Laboratories (Wilmington, MA).
Twelve male Sprague-Dawley rats aged 4 months, weight 448.7 ± 10 grams were obtained locally and housed singly in standard cage conditions.
Fifteen of healthy four-week-old Wistar rats weighing about 200 grams were obtained from Laboratory Animal Facility and Management (LAFAM), UiTM Puncak Alam Campus.
Twelve male Wistar rats (250 300 grams) were obtained from the Animal House of Universiti Kebangsaan Malaysia and used in this study.
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In most instances, a single spot specimen of placenta weighing only a few grams was obtained, without specifying the placental area sampled.
The seeds of horse gram were obtained from Department of Plant Breeding and Genetics, CSK HPKV, Palampur, India.
Determination of PMCA50 based on quadruplicate amplification was also done in our previous study [ 26], and we confirmed that similar PMCA50 values (around 10 per gram) were obtained in two independent studies.
Four bacterial strains, including two Gram-positive as well as two Gram-negative were obtained from the department of Microbiology, Noakhali Science and Technology University, Bangladesh.
The bacterial strains used in this study, Staphylococcus aureus KCTC 1621 (Gram positive) and Escherichia coli KCTC 1682 (Gram negative) were obtained from the Korean Collection for Type Culture (KCTC; Daejeon, Korea).
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