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Liver samples (≈ 0.05 grams per mouse) were allowed to thaw on ice in serum-free DMEM and then cross-linked with 5% formaldehyde for 5 minutes.
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The results were expressed as the number of log10 of viable P. brasiliensis CFUs per gram of tissue per mouse.
This converts atomic units to grams per mole, making the molar mass of hydrogen 1.007 grams per mole, of carbon 12.0107 grams per mole, of oxygen 15.9994 grams per mole and of chlorine 35.453 grams per mole.
Two month-old mice received intraperitoneal injections at 100 µg TM per gram mouse weight once a day for 10 consecutive days and were analysed 3 months later.
Mice (six per group) received either raltegravir (3.28 mg per 20 gram mouse) or maraviroc (1.23 mg per 20 gram mouse) by oral gavage daily.
CCl4 was diluted in mineral oil (Sigma-Aldrich) and administered at a dose of 1 µL per gram of mouse.
Subsequently, the yeast suspension was introduced into the left tail vein, delivering a total of 5×104 yeast cells per gram of mouse.
Mice were first inoculated via the right tail vein with 8ng of RvE1 per gram of mouse or vehicle (<0.1% ethanol) diluted in sterile PBS.
Mice in the DMSO group received 1.5 µl of DMSO per gram of mouse weight at the same time points as the test group.
When tumor diameters were more than 5 mm after 25 days, the 16 tumor-bearing nude mice were randomly divided into two groups: group 1 consisted of eight tumor-bearing nude mice that were intratumorally injected with 0.2 ml normal saline (NS) containing 8 μg cisplatin per gram of mouse body weight for three consecutive days.
In contrast to other Gram-positive pathogens like Streptococcus pneumoniae or group-B streptococci, which are virulent pathogens in humans and mice, mice clear enterococcal bacteremia or peritonitis spontaneously unless a very high inoculum (>108 109 bacteria per mouse) is given.
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CEO of Professional Science Editing for Scientists @ prosciediting.com