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As shown in Fig. 98, within a given class of severity, the grams of tissue undergoing the intratidal collapse did not change significantly between PEEP 5 or 15 cmH2O (63 ± 26 vs 39 ± 32, 92 ± 53 vs 78 ± 142 and 123 ± 94 vs 96 ± 84 in mild, moderate and severe ARDS respectively).
Some results were also expressed in units of grams of tissue.
Approximately 2 grams of tissue from mesenterial lymph nodes were grinded and decontaminated with 2% NaOH.
Roughly one to two grams of tissue was extracted from each sample location and immediately flash frozen in liquid nitrogen.
To prepare samples for nucleotide extraction, 0.5 grams of tissue was homogenized in 10 ml of Trizol LS per sample.
Tissue was cut into approx 2 cm long segments and mixed together before sub-sampling 1 2 grams of tissue for the chitin assay.
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Colony counts were reported as counts per gram of tissue.
A gram of brain tissue takes 20 times more energy to grow and maintain than a gram of tissue from the kidney, heart, or liver, she said.
Gluteal AT-LPL activity (expressed per gram of tissue) significantly increased in all five women by 2.8-fold (1.65±1.30 4.72±3.34 nmol FFA/g/min, P<0.05, Figure 1).
Heparin-releasable LPL activity was measured as previously described.18 AT-LPL activity was expressed as nmol of FFA produced per gram of tissue in 1 min.
Dr. Masoro has found that animals fed a restricted diet burn as much glucose per gram of tissue as do the plumper control animals.
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