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Yield of cells was defined as grams of cells produced per grams of glucose utilized.
Thirty grams of cells were resuspended in 150 mL of buffer A [50 mM HEPES (pH 7.8), 150 mM NaCl, 20 mM imidazole, and 10% glycerol] and disrupted by sonication.
Twenty grams of cells then was incubated with riboflavin (3.8 g, 10.1 mmol) at 30°C for 24 h in 500 ml of 10 mM KPB, pH 7.0, or for 30 h in 100 ml of 10 mM KPB, pH 7.0.
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Biomass levels are expressed as grams of cell weight per milliliter of fermentation broth.
It was expressed in grams of cell dry weight per liter (gCDW/L).
Ten grams of cell paste was sonicated in 50 ml of buffer CBB (50 mM Tris-HCI [pH 7.5], 10% sucrose, 2 mM EDTA, 150 mM KCI, 0.01% NP40, 1 mM DTT, and protease inhibitor cocktail).
In addition, the specific 1,3-PD-producing capability (1,3-PD produced per gram of cells) of the mutant strain was 2-fold that of the parent strain due to a lower growth yield of the mutant.
With this pBAD241 vector, the expression level of the BtsI-1B can reach 50,000 units for each gram of cells.
Caspase-3 activity was calculated as micromolar per gram of cells using a p-nitroaniline calibration curve.
Cells were harvested by centrifugation and cell pellets were resuspended in 5 ml lysis buffer (20 mM Tris HCl, pH 8.0, 150 mM NaCl) per gram of cells.
Cells at different growth phases were harvested and suspended in 2 ml per gram of cells of physiological solution (0.9% (v/v) NaCl).
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