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The seeds of horse gram were obtained from Department of Plant Breeding and Genetics, CSK HPKV, Palampur, India.
Determination of PMCA50 based on quadruplicate amplification was also done in our previous study [ 26], and we confirmed that similar PMCA50 values (around 10 per gram) were obtained in two independent studies.
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Green grams were obtained from the supermarket.
Male Lewis rats weighing approximately 300 grams were obtained from Charles River Laboratories (Wilmington, MA).
Twelve male Wistar rats (250 300 grams) were obtained from the Animal House of Universiti Kebangsaan Malaysia and used in this study.
Twelve, healthy, four-week-old male ICR mice, each weighing about 30 grams, were obtained from Laboratory Animal Facility and Management LAFAMM), Faculty of Pharmacy, UiTM Puncak Alam Campus.
Pacific white shrimp weighing 6 8 grams were obtained from a farm in Chanthaburi province, Thailand, and acclimated at the Aquaculture Business Research Center Laboratory, Faculty of Fisheries, Kasetsart University for 1 week.
Twelve male Sprague-Dawley rats aged 4 months, weight 448.7 ± 10 grams were obtained locally and housed singly in standard cage conditions.
Fifteen of healthy four-week-old Wistar rats weighing about 200 grams were obtained from Laboratory Animal Facility and Management (LAFAM), UiTM Puncak Alam Campus.
Forty male Sprague Dawley (SD) rats at age 8 weeks and ranging from 240 290 grams were obtained from Samtako (Osan, Korea) and were housed in metabolic cages with an automatically controlled temperature (22 ± 2°C), relative humidity (50 60%), and light (12 h light/dark cycle).
Sixty-nine virgin Wistar rats (3 months old) in a body weight range of 250 ± 20.0 grams were obtained from The Experimental Animal Center of the Academy of Military Medical Sciences (Beijing, China) and housed in cages at the experimental animal room at the Institute of Basic Theory of Traditional Chinese Medicine in CACMS.
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