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Buying one of these plants is exactly the same as buying a carved piece of ivory, a tiger skin or a gram of ground rhino horn.
One gram of ground powder was extracted with 3 mL of ethyl acetate by sonication at room temperature for 30 min.
One gram of ground powder was placed into a 15-mL polycarbonate centrifuge tube, and was extracted with 4 mL of ACN-water-acetic acid (79 20 1, v/v/v).
Flax seeds were ground with liquid nitrogen and 10 ml of water was added to every gram of ground flaxseed before application to paper.
Briefly, 0.5 gram of ground leaf tissue was incubated with 5 ml of 95% ethanol at room temperature for 10 min. Extracts were cleared by centrifugation at 12 000 g for 1 min, then diluted 10 times with 95% ethanol.
Each half gram of ground tissue was then inoculated into 92-3TB and 92-3TBL liquid media, mixed, and incubated at 37 degrees Celsius for one to three weeks.
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"The industry standard is 50 grams of ground coffee to one litre of water," Townsend says.
Today, most of the chains use about seven grams of ground coffee for a two-ounce shot.
About 2.5 grams of ground diopside were used in stacked experiments in 0.1 M NaCl solutions buffered using 20 mM sodium borate and HCl.
Two hundred grams of ground plant material were shaken separately in methanol for 48 hrs on an orbital shaker.
Briefly, two hundred milliliters of 70% methanol were added to a beaker containing 10 grams of ground rhizome.
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