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Higher protein content (per gram of cell mass) was observed in complex media containing rich nutrients like yeast extracts than in synthetic media.
Pellets were resuspended in 1.5 mL lysis buffer plus 0.2 U/µl RNasin (Promega) per gram of cell pellet, and then 5 g of cold 0.5 mm glass beads per gram of cell pellet was added.
Lipid content was expressed as grams of lipid per gram of cell mass.
An overall biomass flux of 1.0 thus represents a specific growth rate μ of 1.0 hr -1 (one new gram of cell dry weight per gram of cell dry weight per hour).
The proportion of cellulose as expressed per gram of cell wall was unchanged between the RG and wild type.
The supernatant was removed and the cell pellet was resuspended in 5 mL Bugbuster (Novagen) and 5 μL benzonase (Novagen) per gram of cell pellet.
Similar(44)
In addition, the specific 1,3-PD-producing capability (1,3-PD produced per gram of cells) of the mutant strain was 2-fold that of the parent strain due to a lower growth yield of the mutant.
With this pBAD241 vector, the expression level of the BtsI-1B can reach 50,000 units for each gram of cells.
Caspase-3 activity was calculated as micromolar per gram of cells using a p-nitroaniline calibration curve.
Cells were harvested by centrifugation and cell pellets were resuspended in 5 ml lysis buffer (20 mM Tris HCl, pH 8.0, 150 mM NaCl) per gram of cells.
Cells at different growth phases were harvested and suspended in 2 ml per gram of cells of physiological solution (0.9% (v/v) NaCl).
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